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The serum ferritin concentration is proportional to the amount of iron in stores in the human body. The measurement of serum ferritin enables the clinician to differentiate between the anemia caused by iron deficiency and other forms of anemia. Serum ferritin concentration is a useful, noninvasive screening test for iron overload, which may allow the detection of idiopathic hemochromatosis in the precirrhotic stages.
The enzyme immunoassay for the quantitative determination of serum ferritin is basically a two stage reaction:
STAGE 1: The binding of human serum ferritin to a solid phase antihuman ferritin, and the simultaneous binding of the purified antihuman ferritin conjugated with alkaline phosphatase to the insoluble immune-complex.
STAGE 2: Reaction of alkaline phosphatase with a substrate solution consisting of phenylphosphate disodium and 4-amino-antipyrine. Following the addition of potassium ferricyanide a colour develops, the optical density (490 - 510nm) of which is directly proportional to the ferritin concentration in the sample.
This Kit is Intended for In Vitro Diagnostic Use Only
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WARNING: Reagents in this kit contain sodium azide. Contact with copper or lead drain pipes may result in the formation of explosive azide deposits. It is important during disposal to flush drains with copious amounts of water to prevent azide accumulation. Plumbing that may be contaminated with azides can be flushed with 10 percent sodium hydroxide solution.
Spectro Ferritin Kits
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Kit Contents
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Product Code
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- Prediluted Ferritin Calibrator Solutions: 6 vials containing 0.3ml human spleen ferritin calibrated to concentrations of 6, 20, 60, 200, 600, and 2000ng/ml, against WHO reference material (94/572), in phosphate buffered saline with rabbit serum and sodium azide as a preservative. Store at 2 - 8C.
Do Not Use After Expiration Date on Vial.
- Solid Phase Antihuman Ferritin: 96 microwells ("wells"), in the form of eight
1 X 12 strips, coated with rabbit antihuman spleen ferritin. Stored in a bottle containing borate buffer with bovine serum albumin, rabbit serum, and sodium chloride with sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Bottle.
- Sample Diluting Buffer: 1 bottle containing 20 ml of phosphate buffered saline with rabbit serum and sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Bottle.
- Conjugated Antihuman Ferritin: 1 bottle containing 23 ml of alkaline phosphatase conjugated rabbit antihuman spleen ferritin dissolved in 0.15 M phosphate buffered saline with 5% normal rabbit serum, and sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Bottle.
- Substrate Solution: 1 bottle containing 23 ml of phenylphosphate disodium,
4-amino-antipyrine in 10% diethanolamine with sodium azide as a preservative. Store at 2 - 8C. Do Not Use After Expiration Date on Bottle.
- Potassium Ferricyanide: 1 bottle containing 15 ml of potassium ferricyanide (0.24%) in water.
Store at 2 - 8C. Do Not Use After Expiration Date on Bottle.
- Well Holder: 1 micro well holder.
- Graph Paper: 3 sheets of Spectro Ferritin Standard Plot log-log graph paper.
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S-22
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Spectro Ferritin Kit Availability
