Spectro VwF coated beads

Spectro vWF is an enzyme linked immunoassay (ELISA) for the quantitation of von Willebrand factor (vWF, also called factor VIII-related antigen). This factor is deficient or defective in patients with von Willebrand's disease, a relatively common inherited disorder associated with increased tendency to bleed. Laboratory studies in these patients usually demonstrate:

1. A decrease in procoagulant activity as measured by activated partial thromboplastin time;

2. A prolonged bleeding time; and

3. Decreased plasma concentration of vWF.

A major diagnostic problem concerns the differentiation of patients with von Willebrand disease from those with Classical Hemophilia A (factor VIII coagulant deficiency). Patients with the latter disorder have a decrease in plasma procoagulant activity, i.e. a prolonged activated partial thromboplastin time but have normal or increased concentration of vWF in the plasma. The Spectro vWF assay affords rapid differentiation of these two disorders.

The Spectro vWF assay is also of use in identifying carriers of Hemophilia A. Hemophilia A is a sex-linked disorder; under most circumstances only males have bleeding problems. The affected males inherit the gene for Hemophilia A from mothers who carry the involved gene, but have no bleeding problems.

In order to determine if a woman is a carrier it is necessary to measure factor VIII coagulant activity and vWF concentration. The ratio of factor VIII coagulant activity to vWF concentration in carrier females is approximately 0.5 while the ratio in normal females is 1.0.

This Kit is Intended for In Vitro Diagnostic Use Only

	Copyright Ati Atlas Ltd 2009

WARNING: Reagents in this kit contain sodium azide. Contact with copper or lead drain pipes may result in the formation of explosive azide deposits. It is important during disposal to flush drains with copious amounts of water to prevent azide accumulation. Plumbing that may be contaminated with azides can be flushed with 10 percent sodium hydroxide solution.

A component of this kit contains potassium ferricyanide. Avoid ingestion or contact with skin.

Spectro vWF

Kit Contents

Product Code

Prediluted Human von Willebrand Factor Calibrator Solution: 1 vial containing 2ml of von Willebrand Factor in a concentration equivalent to 50% of the vWF concentration found in the current International Standard for Factor VIII von Willebrand Factor in plasma established by the World Health Organisation. The vWF is dissolved in borate buffer containing bovine serum albumin, rabbit serum, sodium chloride and inert coloring agents with sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Vial.
Solid Phase Antihuman von Willebrand Factor: 64 plastic beads coated with rabbit antihuman vWF stored in borate buffer containing bovine serum albumin, rabbit serum and sodium chloride with sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Vial.
Sample Diluting Buffer: 2 vials each containing 30 ml of borate buffer with bovine serum albumin, sodium chloride, ethylenediaminetetraacetate, inert coloring agents and sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Vial.
Conjugated Antihuman von Willebrand Factor: 1 vial containing 15 ml of alkaline phosphatase conjugated antihuman vWF dissolved in 0.15 M phosphate buffered saline with 5% rabbit serum and sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Vial.
Substrate: 1 vial containing 15 ml of phenylphosphate disodium, 4-amino_antipyrine in 10% diethanolamine with sodium azide as a preservative.
Store at 2 - 8C. Do Not Use After Expiration Date on Vial.
Potassium Ferricyanide (Concentrate): 1 vial containing 20 ml of potassium ferricyanide (2.4%) in water. This Reagent MUST be diluted to a final Volume of 200 ml before use. Store at 2 - 8C. Do Not Use After Expiration Date on Vial.

V-46

Spectro vWF Availability