TfR Kits

• Microwell Strips: 12 (twelve) 1 x 8 microwell strips (96 total wells), pre-coated with polyclonal anti-TfR antibody and stabilized in dry form. The plate is provided in a resealable, ziploc foil pouch containing an inert gas and a drying agent. Store at 2 - 8C.
   
• Plate Sealing Tape: 2 sheets
   
• Microwell Frame: 1
   
• HRP-Conjugate: 1 vial containing 16 ml of Horseradish Peroxidase-conjugated anti-TfR monoclonal antibody and detergent with ProClin 300™ as a preservative. Store at 2 - 8C.
   
• Sample Diluent: 1 vial containing 45 ml of phosphate buffered saline, detergent, and an inert coloring agent with ProClin 300™ as a preservative. Store at 2 - 8C.
   
• Wash Solution 5X Concentrate: 1 bottle containing 50 ml of concentrated phosphate buffered saline and detergent with ProClin 300™ as a preservative. Store at room temperature.
   
• Pre-diluted Standards: 7 vials each containing 0.5 ml of purified TfR protein diluted to concentrations of 0, 10, 20, 40, 100, 200 and 400 ng/ml in phosphate buffer containing BSA, normal rabbit serum, and inert coloring agents with ProClin 300™ as a preservative. Store at 2 - 8C.
   
• Control Sera: 2 vials containing 100 l of undiluted serum, one having a normal level and one having a high level of human transferrin receptors. Store at 2 - 8C.
   
• TMB Substrate Solution A: 1 vial containing 12 ml of a stabilized solution of tetramethylbenzidine (TMB) substrate. Store at 2 - 8C.
   
• TMB Substrate Solution B: 1 vial containing 12 ml of a TMB substrate component in a citric acid buffer containing hydrogen peroxide (H2O2). Store at 2 - 8C.
   
• Acid Stop Solution: 1 bottle containing 10 ml of 2.5 Molar sulfuric acid (H2SO4). Store at 2 -8C.
  Caution: Caustic material. Wear eye, hand, face and clothing protection.
   
• Graph Paper: 4 sheets of 3 cycle log x log graph paper.